| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha : |
29/07/2022 |
|
Actualizado : |
02/09/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
|
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
|
Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
|
DOI : |
10.3389/fvets.2022.889157 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
|
Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
|
Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
|
Asunto categoría : |
-- |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
|
|
Marc : |
LEADER 03260naa a2200325 a 4500
001 1063470
005 2022-09-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fvets.2022.889157$2DOI
100 1 $aGIANNITTI, F.
245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022.
520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations.
653 $aABORTION
653 $aDAIRY CATTLE
653 $aGENETIC DIVERSITY
653 $aMolecular epidemiology
653 $aMultilocus microsatellite typin
653 $aNeospora caninum
653 $aPATHOLOGY
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aREPRODUCTIVE DISEASES
700 1 $aARÁOZ, V.
700 1 $aSILVEIRA, C.S.
700 1 $aFRANCIA, M.E.
700 1 $aROBELLO, C.
700 1 $aCABRERA, A.
773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 50 | |
| 2. |  | SILVEIRA, C.S. Leucosis bovina. In: Jornada de Campo Virtual Porteras Abiertas de Lechería. La Estanzuela, Colonia, Uruguay: INIA, 2020. p.7. (Serie Actividades de Difusión; 795).| Biblioteca(s): INIA La Estanzuela. |
|    |
| 5. | | SILVEIRA, C.S.; CASAUX, L.; ARÁOZ, V.; MACÍAS-RIOSECO, M.; FRAGA, M.; GIANNITTI, F. Abomastitis enfisematosa asociada a Sarcina spp. en una ternera Holstein. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 178-180.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 7. | | RUPPEL, F.; ECHEVERRÍA, S.; GIANNITTI, F.; SILVEIRA, C.S.; ARMENDANO, J.; CABRERA, A.; ROBELLO, C. Desarrollo de un método diagnóstico para la neosporosis bovina. 250. (resúmen) Áreas temáticas: Inmunología. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.166-167. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
| |
| 9. | | SILVEIRA, C.S.; ARÁOZ, V.; CAFFARENA, D.; RIET-CORREA, F.; MIRABALLES, C.; GIANNITTI, F. Brote de intoxicación espontánea por larvas de Perreyia flavipes en un rodeo de bovinos Heredord de Flores, Uruguay. In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 11. | | ARÁOZ, V.; SILVEIRA, C.S.; MORÉ, G.; BANCHERO, G.; RIET-CORREA, F.; GIANNITTI, F. Fatal Sarcocystis cruzi-induced eosinophilic myocarditis in a heifer in Uruguay. Journal of Veterinary Diagnostic Investigation, 2019, Vol. 31(4) 656-660, 2019. Article history: Article first published online: June 10, 2019// Issue published: July 1, 2019| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
|  |
| 13. | | ARÁOZ, V.; SILVEIRA, C.S.; BANCHERO, G.; CABRERA, A.; RIET-CORREA, F.; GIANNITTI, F. Miocarditis granulomatosa eosinofílica asociada a Sarcocystis spp. en una vaquillona de Colonia, Uruguay. In: Jornadas Uruguayas de Buiatría, 45., 2017, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2017. p.156-159.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 16. | | SILVEIRA, C.S.; GIANNITTI, F.; MACIAS RIOSECO, M.; FRAGA, M.; ACOSTA, A.; FRAGA, N.; RIET-CORREA, F. Dermatitis ulcerativa crónica idiopatica en la región distal de los miembros en vacas de cria. In: Jornadas Uruguayas de Buiatría, 44., 2016, Paysandú, UY.; Gianneechini, E.; Elizondo, V. (Ed.).Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2016. p.171-173| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 17. | | SILVEIRA, C.S.; GRILLI, M.J.; BERON, M.; CAFFARENA, D.; FRAGA, M.; GIANNITTI, F.; FERNÁNDEZ-CIGANDA, S. Detecção e quantificação do vírus da leucose bovina em terneiros neonatos de estabelecimentos leiteiros do Uruguai. [resumo] In: Encontro Nacional de Diagnóstico Veterinário (ENDIVET), 11, Resumos, Cuiabá, Mato Grosso, Brazil, Novembro 21-23, 2022.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
| |
| 18. | | SILVEIRA, C.S.; FRAGA, M.; MONESIGLIO, M.C.; DELPIAZZO, R.; MACÍAS-RIOSECO, M.; GIANNITTI, F.; RIET-CORREA, F. Detección de Tritrichomonas foetus por PCR en esmegma prepucial de toros en Uruguay. [Tritrichomonas foetus by PCR in preputial smegma of bulls in Uruguay]. Veterinaria (Montev.), 56 (213), Ene-Jun 2020, e20205621307. DOI: https://doi.org/10.29155/VET.56.213.7 Article history:Recibido: 06/05/19/Aceptado: 18/03/19| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
| |
| 19. | | SLIMOVICH, L.; CRESCIONINI, A.; SALADA, S.; SILVEIRA, C.S.; FERNÁNDEZ-CIGANDA, S.; DHUNGYEL, O.; FIERRO, S.; FRAGA, M. Determinación de serogrupos circulantes de Dichelobacter nodosusen majadas con diagnóstico clínico de Foot-Rot en ovinos de Uruguay. Resultados Preliminares. [abstract]. In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Una Sola Salud (Single Health Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.99-102. (Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) Corresponding author: L. Slimovich, Plataforma de Investigación en Salud Animal, INIA-La Estanzuela, mailto:ludmilaslimovich@gmail.com| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
| |
| 20. | | SILVEIRA, C.S.; ARMENDANO, J.I.; MOORE, D.P.; CANTÓN, G.J.; MACÍAS-RIOSECO, M.; RIET-CORREA, F.; GIANNITTI, F. A comparative study of commercial ELISAs for antibody detection in the diagnostic investigation of Neospora caninum-associated abortion in dairy cattle herds in Uruguay. [Comparación de ELISAs comerciales para la detección de anticuerpos en la investigación diagnóstica del aborto asociado a Neospora caninum en rodeos lecheros de Uruguay]. Revista Argentina de Microbiología, April - June 2020, Volume 52, Issue 2, Pages 107-114. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2019.06.004 Article history: Recibido 01 marzo 2019. / Aceptado 24 junio 2019. / Disponible online 28 de Noviembre de 2019.
Corresponding author: da Silva Silveira, C.; Instituto Nacional de Investigación Agropecuaria (INIA), Plataforma de...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
|  |
| Registros recuperados : 50 | |
|
| Expresión de búsqueda válido. Check! |
|
|
